RNA Therapeutics Platform Staff Scientist

We are looking for a talented research assistant to participate in the development of RNA Therapeutics at the Sidney-Altman Hub at the Montreal Clinical Research Institute (IRCM).

You will play a central role in establishing the platform services and in developing different modalities of RNA therapeutics (siRNA, ASO, PMO, mRNA-LNP). This position offers a unique opportunity to contribute to cutting-edge research in RNA therapies and to stimulate innovation in RNA biology.

Summary of responsibilities

Project management and execution:

• Perform research design and planning of RNA therapeutic projects together with the platform manager and scientific director.
• Carry out laboratory work, including:
  • Synthesis, purification and NGS of sc-pDNA plasmids and vectors
  • Design of RNA sequences and vectors
  • In vitro RNA synthesis, purification, and associated quality control assays using HPLC, Nandrop, ELISA, Flow Cytometry, etc.
  • Analysis of quality of DNA templates such as Poly A tail, linearization efficiency, and quantification.
  • Quality control and analysis of RNA using Nanodrop, ELISA, nanopore sequencing
  • Formulation of lipid nanoparticles and RNA encapsulation, and quality control monitoring
  • Screening in cell culture test dose-response effects
  • Assist with synthesis of oligonucleotides, e.g. ASO, siRNA, PMO, sgRNA including high throughput containing standard and/or modified nucleobases, as well as purification, lyophilization, and analysis thereof.
  • Apply a wide range of molecular biology techniques and core lab skills and develop and/or optimize experimental procedures, e.g., for designing, identification, quantification, and purification of plasmid DNA and PCR amplified templates, plasmid linearization, bioconjugation, etc.
• Create, collect and interpret experimental data with strict adherence to data integrity according to GLP guidelines.
• Assist in establishing a quality control measure to ensure the highest standards in RNA, oligonucleotide and nanolipid particle synthesis and purification processes, screening, cell culture assays, and in vivo functional assays.

Operational and collaboration:

• Contribute to the development of the service offering of the RNA therapeutic platform with the manager.
• Support collaborative interactions with partner laboratories, institutions and clients.

Required qualifications:

• At least a Master’s degree (MSc) in molecular biology, biochemistry, bioengineering or a related field.
• Two-three years industry experience.
• Experience and skills in cell culture and cell-based assays, PCR (various), in vitro RNA synthesis, HPLC, FPLC, Mass Spectrometry, Capillary Electrophoresis, Lipid nanoparticles, DLS, UF/DF, Nanodrop 
• Strong background in mammalian and bacterial cell culture, recombination and transformation, broad range of molecular biology techniques such as cloning, amplification, transcription, translation, and associated assays and bioanalytical techniques including method development and qualification.

Knowledge and skills:

• Previous exposure to therapeutic RNA applications.
• Demonstrated knowledge of fundamental principles of RNA biology.
• Superior computer literacy: MS-Office and planning tools, i.e. Smart Sheets, Project, Excel (PivotTable, data trending, etc.)
• Strong analytical, problem-solving and communication skills.
• Excellent organizational and documentation skills.
• Strong and the ability to multitask and prioritize effectively.
• General knowledge of chemical synthesis of oligonucleotide and ligation.
• Understanding of cGMP (Good Manufacturing Practice) requirements.
• Exposure to LIMS and QMS.

Working conditions

• Full-time temporary position (35 hours/week) with the possibility of extension.
• Prime location: Offices near Mount Royal Park, Saint-Laurent Boulevard, and the Quartier des Spectacles, just a 10-minute walk from the metro.
• Compensation and benefits: Competitive salary based on experience and skills, aligned with market standards.
• RREGOP pension plan: Enjoy a generous retirement plan to help secure and plan your future.
• Additional benefits: Medical insurance, telemedicine, and an Employee Assistance Program (EAP).
• Vacation: Annual paid vacation to support a healthy work-life balance.